Single Particle Tracking (SPT)

• Mode of action: detection of fluorescently labeled nanoparticles in buffer, extracellular fluids and inside living cells. 
• Input: nanomolar concentrations of fluorescent nanoparticles (Ex 488 or Ex 647), 5-50 µl per sample, or cells seeded on glass bottomed dish (around 150 µm thick). 
• Goal: diffusional properties, concentration measurements, nanoparticle aggregation in biofluids
• Applications related to cancer: assess the stability of nanoparticles containing nucleic acids in the presence of biofluids such as serum and human ascites fluid. 
• Advantage: sensitive and fast readout, > 15 years of experience   

Faculty of Pharmaceutical Sciences, Ottergemsesteenweg 460, 9000 Gent
 

responsible PI's and contact persons: prof. Katrien Remaut & prof. Kevin Braeckmans