prof. Evelyne Meyer (PhD)

The research mission of the Laboratory of Biochemistry is to contribute to the elucidation of inflammatory processes such as mammary tumor progression and metastasis, using a fully characterised mouse intraductal inoculation model for triple-negative breast cancer. For this purpose several wild type strains as well as genetically modified (e.g. NF-kB reporter) mice are used and 4T1-luc cells are typically inoculated although Py230 cells are an alternative option. This preclinical model is also an elegant screening tool for novel candidate therapeutic molecules such as c-Met inhibitors in comparison to classical chemotherapy (cisplatin). In this context tumor-associated macrophages and neutrophils as key innate immune cells are typically analysed and cytokine profiling as well as IVIS are performed. Complementing this inflammatory profile, chitinase 3-like 1 and lipocalin-2 are evaluated as a novel innate immunity related protein biomarkers for inflammation and metastasis.
The Laboratory of Biochemistry has developed an extensive set of more than 150 standard operation procedures (e.g. for immunohistochemistry, 3-laser flowcytometry, multiplex, cell culture, western blotting, ELISA) to analyze a variety of biological samples such as serum, mammary tumor, lymph node, spleen, lung tissue, and cell culture supernatant obtained from in vivo and in vitro experimental studies.
 

• dr. Jonas Steenbrugge (PhD) - post-doctoral fellow
• Anna Blockeel - doctoral fellow
• Robbe Salembier - doctoral fellow
• Caro De Haes - doctoral fellow
• Kristel Demeyere - laboratory coordinator
   

• Comparative Profiling of Metastatic 4T1- vs. Non-metastatic Py230-Based Mammary Tumors in an Intraductal Model for Triple-Negative Breast Cancer. Frontiers in Immunology, 2019.
• Selective pharmacological inhibitors of HDAC6 reveal biochemical activity but functional tolerance in cancer models. INTERNATIONAL JOURNAL OF CANCER, 2019.
• Mononuclear but Not Polymorphonuclear Phagocyte Depletion Increases Circulation Times and Improves Mammary Tumor-Homing Efficiency of Donor Bone Marrow-Derived Monocytes. Cancers, 2019.
• Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2018. 
• Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis. CLINICAL & EXPERIMENTAL METASTASIS, 2018.
• Off-target and tumor-specific accumulation of monocytes, macrophages and myeloid-derived suppressor cells after systemic injection. NEOPLASIA, 2018.
• Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer. JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 2016
   

• Lab address: Salisburylaan 133, 9820 - Merelbeke
• Can provide specific platforms or services for other researchers: Immunohistochemical tumor microenvironment characterization (mouse), flow cytometric immunophenotyping, multiplex cytokine profiling 
• The department of Veterinary and Biosciences 
• LinkedIn
• Provaxs 
• Belgian Society for Advancement of Cytometry (BSAC)
• prof. Meyer is involved in Animal facility and L2 lab for preclinical cancer research, Laboratory of Biochemistry, Faculty of Veterinary Medicine
• prof. Meyer is interested to receive invitations for talks or presentations