epigenomics & transcriptomics

Over the recent years, the lab of prof. Frank Speleman has significantly invested in the implementation of novel next-generation sequencing based techniques to study the epigenome and the transcriptome at various levels. We perform ChIP-sequencing for various histone modifications (H3K4me1/3, H3K27ac/me3) and various transcriptional regulators, apply ATAC-seq to identify open chromatin regions and 4C-sequencing to identify 3-dimensional regulatory interactions between genomic regions. Transcriptome analysis is now standardly performed using RNA-sequencing (both polyA and total), with in house library preparation and sequencing. Given the growing role for enhancers in gene regulation, we are currently exploring the possibilities of the GRO-seq method.

• MRB-UZ, Corneel Heymanslaan 10, 9000 Gent

• Speleman lab