Single cell platforms

Studying cancer cells at the individual level provides a powerful new approach to study complex biological processes and large scale heterogeneity in human cancers. Within CRIG, following state-of-the-art platforms enable the study of single cancer cell (epi)genomes, transcriptomes, targeted proteomes, morphologies and behavior:

• IncuCyte (Essen Biosciences) - live cell imaging platform for cellular and phenotypic screening

• Laser Capture Microdissection (LCM)

• MACSima: spatial targeted multi-omics

• MILO Single-Cell Western (Bio-Techne) - Western analysis on single cell level

• MicroScale Thermophoresis (NanoTemper)

• Operetta (Perkin Elmer) - high content cellular screening platforms

• Vycap - Single (rare) cell isolation and identification

In addition, VIB has a single cell core facility offering a one-stop solution for single cell analyses. The platforms provides access to state-of-the-art services.

following teams have specific expertise in single cell analysis:

• single cell RNA-sequencing analysis & data-mining pipeline (contact Prof. Katleen De Preter)
• single cell ATAC and CITE sequencing analysis & data-mining pipelines (contact Prof. Katleen De Preter & Prof. Celine Everaert)