Single cell platforms
Studying cancer cells at the individual level provides a powerful new approach to study complex biological processes and large scale heterogeneity in human cancers. Within CRIG, following state-of-the-art platforms enable the study of single cancer cell (epi)genomes, transcriptomes, targeted proteomes, morphologies and behavior:
- C1 (Fluidigm) - Single Cell Auto Prep system for genome and transcriptome sequencing
- QX100 droplet digital PCR (Bio-Rad) - read more - contact: prof. Jo Vandesompele
- IncuCyte (Essen Biosciences) - live cell imaging platform for cellular and phenotypic screening
- Laser Capture Microdissection (LCM)
- MILO Single-Cell Western (Bio-Techne) - Western analysis on single cell level
- MicroScale Thermophoresis (NanoTemper)
- Operetta (Perkin Elmer) - high content cellular screening platforms
- DEPArray Nxt (Silicon Biosystems) - identification, quantification and recovery of rare individual cells
- ddSEQ Single-Cell Isolator (Biorad) - robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a single experiment
- Vycap - Single (rare) cell isolation and identification
In addition, VIB has launched the Singularity platform offering a one-stop solution for single cell analyses. The platforms provides access to state-of-the-art flow cytometry, single cell RNA sequencing and advanced data processing workflows. They offer:
Package services
- Consultation experimental design and planning
- Single cell isolation and sorting
- Library preparation (10X genomics)
- Sequencing
- Data processing
- Basic analysis, quality reports and customized analysis tools
- Bio-informatic training and support
Add-on services
- Immune profiling
- Cell hashing
- CITE-seq
- Advanced bio-informatic
- Analysis and data-mining