5200 Fragment Analyzer (Agilent)

• Mode of action: capillary electrophoresis to determine nucleic acid fragment length and concentration
• 2.5 µL sample input needed of DNA (5-500 picogram/µL) or RNA (2.5 ng/µL). 
• Using the DNA kit (50-1500 bp max):
  -    electropherogram with determination of length, concentration, and optional smear analysis
• Using the RNA kit: 
  -    quality of the sample (RIN value)
  -    analysis of RNA fragment length and concentration
• The instrument has 12 capillaries, and a run takes 1 hour for all 12 samples (11 samples and one ladder); an entire 96-well plate can be loaded for overnight analysis
• A core team operates the instrument, and samples can be analyzed in service at cost (for Ghent University researchers). Make an appointment via fragmentanalyzercyto@cmgg.be .
   

Read more about the instrument on the manufacturer's website

campus UZ Gent, MRB1 (entrance 34, room 100.028), Corneel Heymanslaan 10, 9000 Gent

• prof. Jo Vandesompele