Covaris sonicator S220

Platforms & Expertise

accessible for academic partners

Description

Mode-of-action: this device can be used for DNA fragmentation in a reproducible manner. It works through the ‘adaptive focused acoustics’ technology, referring to the use of a focused acoustic wave that leads to a mechanical energy inferred on the sample for shearing in an isothermal way.
Input: can be either purified DNA or in case of ChIP-seq samples purified and lysed nuclei
Output: reproducible DNA fragmentation of a uniform length of 200-300 bp ideal for library prep
In our research team this is a standard part of the procedure followed for ChIP-sequencing
The advantages compared to other sonicators is that a focused beam onto the sample is generated leading to minimal heating of the sample and very reproducible results

MRB1, UZ campus, Corneel Heymanslaan 10, 9000 Gent