Lipidomics platform
Accessible to academic and industrial partners
Description
The Laboratory of Integrative Metabolomics (LIMET), led by Prof. Lynn Vanhaecke, has established expertise in developing and applying advanced mass spectrometry-based omics methods for small molecules, including (semi)quantitative, targeted, and untargeted approaches, to address research questions related to food safety, public health, and individual well-being.
Lipids, alongside the polar fraction of the metabolome, are essential biomolecules involved in numerous biological functions. Dysregulated lipid metabolism is a hallmark of various pathological conditions, underscoring its critical role in disease mechanisms. LIMET has established and validated UHPLC-HRMS lipidomics platforms incorporating Thermo Fisher Q-Exactive and Exploris 120 mass spectrometers, coupled with single and dual UHPLC pump configurations, respectively. The dual-pump system enables the simultaneous acquisition of metabolomic and lipidomic data on a single platform. This methodology enables comprehensive lipid profiling, providing coverage across all eight major lipid classes - fatty acyls, glycerolipids, phosphoglycerolipids, polyketides, prenols, saccharolipids, sphingolipids, and sterols. It supports the analysis of biofluids (e.g., feces, plasma, urine, saliva), tissues, and cell line samples, with potential extensions to other biological matrices, including microbial cultures.
CRIG members and other interested researchers are welcome to inquire about sample requirements, collaborative opportunities, and analysis costs.
Location
Department of Veterinary Public Health and Food Safety, Laboratory of Integrative Metabolomics, Salisburylaan 133, B-9820 Merelbeke