prof. Evelyne Meyer (PhD)

CRIG group leader
Evelyne Meyer


Principal investigator - Lab of Biochemistry; Department of Pharmacology, Toxicology and Biochemistry (UGent)
Full professor (Faculty of Veterinary Medicine, UGent)

 

Research focus

The research mission of the Laboratory of Biochemistry is to contribute to the elucidation of inflammatory processes such as mammary tumor progression and metastasis, using a fully characterised mouse intraductal inoculation model for triple-negative breast cancer. For this purpose several wild type strains as well as genetically modified (e.g. NF-kB reporter) mice are used and 4T1-luc cells are typically inoculated although Py230 cells are an alternative option. This preclinical model is also an elegant screening tool for novel candidate therapeutic molecules such as c-Met inhibitors in comparison to classical chemotherapy (cisplatin). In this context tumor-associated macrophages and neutrophils as key innate immune cells are typically analysed and cytokine profiling as well as IVIS are performed. Complementing this inflammatory profile, chitinase 3-like 1 and lipocalin-2 are evaluated as a novel innate immunity related protein biomarkers for inflammation and metastasis.
The Laboratory of Biochemistry has developed an extensive set of more than 150 standard operation procedures (e.g. for immunohistochemistry, 3-laser flowcytometry, multiplex, cell culture, western blotting, ELISA) to analyze a variety of biological samples such as serum, mammary tumor, lymph node, spleen, lung tissue, and cell culture supernatant obtained from in vivo and in vitro experimental studies.
 

Research team

Key publications

  • Comparative Profiling of Metastatic 4T1- vs. Non-metastatic Py230-Based Mammary Tumors in an Intraductal Model for Triple-Negative Breast Cancer. Frontiers in Immunology, 2019.
  • Selective pharmacological inhibitors of HDAC6 reveal biochemical activity but functional tolerance in cancer models. INTERNATIONAL JOURNAL OF CANCER, 2019.
  • Mononuclear but Not Polymorphonuclear Phagocyte Depletion Increases Circulation Times and Improves Mammary Tumor-Homing Efficiency of Donor Bone Marrow-Derived Monocytes. Cancers, 2019.
  • Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2018. 
  • Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis. CLINICAL & EXPERIMENTAL METASTASIS, 2018.
  • Off-target and tumor-specific accumulation of monocytes, macrophages and myeloid-derived suppressor cells after systemic injection. NEOPLASIA, 2018.
  • Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer. JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 2016
     

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