prof. Kris Gevaert (PhD)

CRIG member
Kris Gevaert

Full professor (Faculty of Medicine and Health Sciences, UGent)
Group leader (Medical Biotechnology Center, VIB-UGent)
Associate department director (Medical Biotechnology Center, VIB-UGent)
Scientific advisor (Proteomics Expertise Center, VIB-UGent)

Research focus

As proteins are among the main molecular players in cells, their in-depth study is important for cancer research as in cancers, proteins are frequently deregulated or mutated. My group has introduced and applied novel technologies for comprehensive analysis and quantification of cellular proteins; the proteomes. We focus our proteome studies on the analysis of several protein modifications such as protein phosphorylation by kinases, protein ubiquitination and protein processing by proteases. We use high-end mass spectrometers for identifying proteins with deregulated levels among different proteomes, allowing us to spot potential protein biomarkers. Classical and novel affinity purification-mass spectrometry (AP-MS) approaches are used to map the composition of protein complexes in which most, if not all, proteins reside and exert their functions. We master multiple reaction monitoring (MRM) approaches for quantifying proteins in samples, in which a mass spectrometer thus replaces a Western blot device. We are interested in protein biogenesis and combine ribo-seq data with positional proteomics data to map different N-terminal proteoforms and try to understand their functions. More recently, we implemented thermal proteome profiling proteomics for identifying the targets (and off-targets) of small molecule drugs.

Research team

  • prof. Kris Gevaert - group leader, full professor
  • Jasmine Barra - doctoral fellow
  • Ludger Goeminne - doctoral fellow
  • Tijs Merckaert - doctoral fellow
  • Jarne Pauwels - technician
  • Delphi Van Haver - technician
  • An Staes - technician

Key publications

  • Trapping mammalian protein complexes in viral particles’. Nature Communication, 2016. (PMID: 27122307)
  • 'Melanoma addiction to the long non-coding RNA SAMMSON’. Nature, 2016. (PMID: 27008969)
  • ‘Limited proteolysis combined with stable isotope labelling reveals conformational changes in protein (pseudo)kinases upon binding small molecules’. Journal of Proteome Research, 2015. (PMID: 26293246)
  • 'Proteomic identification of cysteine cathepsin substrates shed from the surface of cancer cells’. Molecular & Cellular Proteomics, 2015. (PMID: 26081835)
  • 'NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth inhibitory effects by inducing a G1 cell cycle arrest’. BMC Cancer, 2015. (PMID: 25958384)
  • 'Structural Investigation of B-Raf Paradox Breaker and Inducer Inhibitors’. Journal of Medicinal Chemistry, 2015. (PMID: 25611072)
  • ‘Early targets of miR-34a in neuroblastoma’. Molecular & Cellular Proteomics, 2014. (PMID: 24912852)
  • ‘A nanobody targeting the F-actin capping protein CapG restrains breast cancer metastasis’. Breast Cancer Research, 2013. (PMID: 24330716)
  • ‘The miR-17-92 microRNA cluster regulates multiple components of the TGF-β pathway in neuroblastoma’. Molecular Cell, 2010. (PMID: 21145484)