In vitro and in vivo models for studying hematopoiesis
Accessible for academic and industrial partners
To model hematopoietic stem cell differentiation into the various hematopoietic lineages, MS5 and OP9 based stromal cocultures are available to direct development into erythroid, myeloid and lymphoid (B, T and NK) lineages. For T cell development, OP9-DLL1 (or OP9-DLL4) cocultures, fetal thymus organ cultures (FTOCs, also with thymic lobes from gene modified mice) or organoid thymic organ cultures (ATOs) can be used. We also have experience with in vivo reconstitution models (NSG mice, intrahepatic injection in newborns or intravenous injections in tail vein). All these experiments can be performed with hematopoietic precursor cells from mice or human, and can be combined with retro- or lentiviral transductions for overexpression or knockdown studies to study the function of potential oncogenes or tumor suppressor genes.
UZ Gent, Corneel Heymanslaan 10, MRB2, Ingang 38, 9000 Gent
Responsible PI and contact person: prof. Tom Taghon